FIGURE

Fig. 4

ID

ZDB-FIG-190527-2

Publication

Zheng et al., 2017 - Spexin Suppress Food Intake in Zebrafish: Evidence from Gene Knockout Study

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Fig. 4

Gene expression analysis of neuropeptides and receptors in the hypothalamus in response to feeding. Quantitative PCR analysis is used to measure mRNA levels of AgRP1 and NPY (upper panel), POMC1 and CART1 (middle panel), GalR2a and GalR2b (lower panel) in WT and spx1 −/− zebrafish at 1 h, 0 h before feeding, 1 h and 3 h after feeding. Expression of elongation factor 1 alpha is used to normalize all samples. All relative expressions are represented as the ratio to the 0 h level respectively. Data were obtained in triplicate and are represented as mean ± s.e.m. (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001 by two-way ANOVA with Bonferroni’s post hoc test.

Expression Data

Genes:	agrp cart1 galr2a galr2b npy pomca (all 6)
Fish:	WT spx^{zf2090/zf2090} (AB)
Anatomical Term:	hypothalamus
Stage:	Adult

Expression Detail

Gene	Fish	Conditions	Stage	Anatomy	Assay
agrp	WT	standard conditions	Adult	hypothalamus	RTPCR
agrp	spx^{zf2090/zf2090} (AB)	standard conditions	Adult	hypothalamus	RTPCR
cart1	WT	standard conditions	Adult	hypothalamus	RTPCR
cart1	spx^{zf2090/zf2090} (AB)	standard conditions	Adult	hypothalamus	RTPCR
galr2a	WT	standard conditions	Adult	hypothalamus	RTPCR
galr2a	spx^{zf2090/zf2090} (AB)	standard conditions	Adult	hypothalamus	RTPCR
galr2b	WT	standard conditions	Adult	hypothalamus	RTPCR
galr2b	spx^{zf2090/zf2090} (AB)	standard conditions	Adult	hypothalamus	RTPCR
npy	WT	standard conditions	Adult	hypothalamus	RTPCR
npy	spx^{zf2090/zf2090} (AB)	standard conditions	Adult	hypothalamus	RTPCR

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Antibody Labeling

No data available

Phenotype Data

Fish:	spx^{zf2090/zf2090} (AB)
Observed In:	hypothalamus
Stage:	Adult

Phenotype Detail

Acknowledgments

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