FIGURE

Fig. 6

ID

ZDB-FIG-190328-8

Publication

Cao et al., 2018 - Regional Gene Expression Profile Comparison Reveals the Unique Transcriptome of the Optic Fissure

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Fig. 6

afap1l2 controls OF closure by regulating pax2a expression. (A–F) ISH of pax2a in the eyes of control, afap1l2-morphant, and afap1l2-overexpressing zebrafish at 36 hpf. (A, B) Lateral views; (C, D) ventral views. (G) qPCR comparison of afap1l2 expression levels in the eyes of wild-type and pax2a-morphant zebrafish. FC: fold change. (H) Quantification of incidences of coloboma phenotype of afap1l2-MO and pax2a-MO coinjection. Numbers in parentheses denote dosage of the morpholinos used and the number of zebrafish injected. The scale bars in (E, F) are both 100 μm, and they apply to (A, C, E) and to (B, D, F), respectively.

Expression Data

Genes:	afap1l2 pax2a
Fish:	AB AB + MO2-afap1l2 AB + MO4-pax2a
Knockdown Reagents:	MO2-afap1l2 MO4-pax2a
Anatomical Terms:	eye eye ventral region optic furrow
Stage:	Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO2-afap1l2 AB + MO4-pax2a AB + MO2-afap1l2 + MO4-pax2a
Knockdown Reagents:	MO2-afap1l2 MO4-pax2a
Observed In:	eye ventral region optic furrow
Stage Range:	Prim-25 to Protruding-mouth

Phenotype Detail

Acknowledgments

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