FIGURE

Fig. 4

ID

ZDB-FIG-190129-17

Publication

Dingare et al., 2018 - The Hippo pathway effector Taz is required for cell morphogenesis and fertilization in zebrafish

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Fig. 4

Taz protein is a bona fide marker of the MC. (A-E) Confocal images of stage III WT oocytes stained with anti-Taz (A) and anti-β-catenin (C) antibodies, and DAPI (B, n=4). D shows the three channels merged, and E is an orthogonal view of D. (F-G′) Stage III buc mutant oocytes stained with the anti-Taz antibody and DAPI (n=4). G and G′ are orthogonal views of F. (H-P) Confocal images of wholemount Tg(4xGTIIC:d2egfp)^mw50/0 oocytes stained with the anti-Taz antibody (magenta), ccnb1 mRNA (red) and DAPI (blue) at early stage II (H-J), mid-stage II (K-M) and late stage II (N-P) (n=38). Hippo reporter activity is shown in green. Cyan brackets in orthogonal views (M,P) indicate the gap between the oocyte surface and the FC layer (microvilli are not visible); white arrows indicate an MC; dotted arrowheads (I,L) point to non-follicle cells. Insets in L,L′,O,O′ show higher magnification of center of panel. Scale bars: 20 μm in A-D,F,I,J,L,M,O,P; 40 μm in H,K,N.

Expression Data

Genes:	ccnb1 d2EGFP wwtr1
Antibodies:	Ab1-ctnnb Ab1-wwtr1
Fish:	WT buc^{p106re/p106re} mw50Tg
Anatomical Terms:	micropylar cell oocyte stage II anterior region oocyte stage II micropylar cell oocyte stage III anterior region oocyte stage III micropylar cell ovarian follicle lateral plasma membrane
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	buc^{p106re/p106re}
Observed In:	micropylar cell ovarian follicle stage III
Stage:	Adult

Phenotype Detail

Acknowledgments

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