FIGURE

Fig. 3

ID

ZDB-FIG-190129-16

Publication

Dingare et al., 2018 - The Hippo pathway effector Taz is required for cell morphogenesis and fertilization in zebrafish

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Fig. 3

wwtr1 mutant follicles lack the micropylar cell. (A-P) Maximum intensity projection of confocal images of wwtr1^+/+ (A-H, n=31) or wwtr1^−/− (I-P, n=40) follicles stained by ISH to detect ccnb1 mRNA at the animal pole (red), combined with DAPI nuclear staining (blue) at the indicated oocyte stages. (D,L,H,P) Orthogonal views of corresponding confocal stacks (C,K,G,O). (Q-R) Orthogonal views of confocal stacks of wwtr1^+/− (Q, n=9) or wwtr1^−/− (R, n=7) follicles stained with Rhodamine Phalloidin. Green arrows indicate an MC; red arrows indicate the indentation of the oolemma; yellow brackets indicate the FCL; green brackets indicate the gap between the FCL and the oocyte surface in wwtr1^−/− follicles. The zp0.5:egfp-zorba transgene (not shown) was used to localize the animal pole and orient the follicles. Scale bars: 20 μm.

Expression Data

Gene:	ccnb1
Fish:	WT wwtr1^fu55/fu55
Anatomical Terms:	oocyte stage II anterior region oocyte stage III anterior region
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	wwtr1^fu55/fu55
Observed In:	micropylar cell oocyte stage II chorion micropyle formation oocyte stage III chorion micropyle formation ovarian follicle stage II ovarian follicle stage III
Stage:	Adult

Phenotype Detail

Acknowledgments

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