FIGURE

Fig. 2

ID

ZDB-FIG-190118-2

Publication

Gutzman et al., 2018 - Basal constriction during midbrain-hindbrain boundary morphogenesis is mediated by Wnt5b and focal adhesion kinase

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Fig. 2

Wnt5b regulates basal constriction possibly through Gsk3β. (A–D) In situ hybridization of wnt5b expression during MHB development at 18 ss (A), 22 ss (B), and prim-6 (C). (D) prim-6 sense probe control. (E–G′) Live confocal images of the MHB region in prim-6 stage embryos. Single-cell wild-type embryos were injected with mGFP to label cell membranes and co-injected with control MO (E,E′), wnt5b MO (F,F′), or wnt5b MO and dnGsk3β mRNA (G,G′). (H) Quantification of basal cell width in control MO, wnt5b MO, dnGsk3β mRNA (image not shown), and wnt5b MO+dnGsk3β mRNA injected embryos. (H) For each treatment group, n=3 embryos. For each embryo, 6 cells located at the MHBC were measured, 3 cells on each side. Arrowheads indicate MHBC. M, midbrain; H, hindbrain. Scale bars: 26 µm.

Expression Data

Gene:	wnt5b
Fish:	AB
Anatomical Term:	midbrain hindbrain boundary constriction
Stage Range:	14-19 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO2-wnt5b + MO4-tp53
Knockdown Reagents:	MO2-wnt5b MO4-tp53
Observed In:	midbrain hindbrain boundary constriction basal region midbrain hindbrain boundary constriction epithelial cell morphogenesis midbrain hindbrain boundary constriction midbrain-hindbrain boundary morphogenesis
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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