FIGURE

Fig. S2

ID

ZDB-FIG-190118-6

Publication

Gutzman et al., 2018 - Basal constriction during midbrain-hindbrain boundary morphogenesis is mediated by Wnt5b and focal adhesion kinase

Other Figures

All Figure Page

Back to All Figure Page

Fig. S2

Gross morphology images of dnGsk3 and wild-type Gsk3 overexpression phenotypes and dnGsk3 rescue of wnt5b morphants.

(A-D) Brightfield dorsal and lateral images of control MO (A,B) and wnt5b MO (C,D) injected embryos, co-injected with mGFP (A,C), or dnGsk3 (B,D) mRNA. (A) Control morphants co-injected with mGFP mRNA demonstrating a normal MHBC basal constriction phenotype. (B) Control morphants co-injected with dnGsk3 mRNA exhibit an eyeless phenotype, but undergo basal constriction normally with this concentration of dnGsk3 (n=9). (C) Wnt5b morphants co-injected with control mRNA exhibit abnormal MHBC morphogenesis, tail defects, and fail to undergo basal constriction (n=6). (D) Wnt5b morphants co-injected with dnGsk3 mRNA exhibit a loss of eyes and tail defects, but the gross morphology of basal constriction is rescued and occurs normally (n=6). Arrowheads indicate the MHBC. Scale bars: 100m.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biol. Open