FIGURE

Fig. 7

ID

ZDB-FIG-181009-13

Publication

Zhang et al., 2018 - Distant Insulin Signaling Regulates Vertebrate Pigmentation through the Sheddase Bace2

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Fig. 7

Insulin b Is the Upstream Ligand that Drives Melanophore Dendricity in Wanderlust

(A and B) Morpholino knockdown of the ligand insulin B (insb) in bace2^−/− embryos rescues the hyperdendritic melanophores, quantified in (B). The data are from three independent experiments, two-tailed t test, ^∗∗∗∗p < 0.0001.

(C–E) Fluorescent ISH shows zebrafish insb ligand mRNA is initially expressed ubiquitously at 24 hpf and then restricted to the head/brain region (arrowhead) in bace2^−/− embryos at 48–72 hpf, sense probe controls for background signal. All bar graphs are presented as mean ± SEM. Scale bars: (A) 100 μm, (C and D) 150 μm, and (E) 200 μm.

See also Figures S5 and S7.

Expression Data

Gene:	insb
Fish:	AB AB + MO1-bace2
Knockdown Reagent:	MO1-bace2
Anatomical Terms:	head anatomical region whole organism
Stage Range:	Prim-5 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-bace2 bace2^{hu3332/hu3332} + MO2-insb
Knockdown Reagents:	MO1-bace2 MO2-insb
Observed In:	caudal fin melanocyte
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 45(5), Zhang, Y.M., Zimmer, M.A., Guardia, T., Callahan, S.J., Mondal, C., Di Martino, J., Takagi, T., Fennell, M., Garippa, R., Campbell, N.R., Bravo-Cordero, J.J., White, R.M., Distant Insulin Signaling Regulates Vertebrate Pigmentation through the Sheddase Bace2, 580-594.e7, Copyright (2018) with permission from Elsevier. Full text @ Dev. Cell