ZFIN Image: Zhang et al., 2018, Fig. 7

Image

Figure Caption

Fig. 7

Insulin b Is the Upstream Ligand that Drives Melanophore Dendricity in Wanderlust

(A and B) Morpholino knockdown of the ligand insulin B (insb) in bace2^−/− embryos rescues the hyperdendritic melanophores, quantified in (B). The data are from three independent experiments, two-tailed t test, ^∗∗∗∗p < 0.0001.

(C–E) Fluorescent ISH shows zebrafish insb ligand mRNA is initially expressed ubiquitously at 24 hpf and then restricted to the head/brain region (arrowhead) in bace2^−/− embryos at 48–72 hpf, sense probe controls for background signal. All bar graphs are presented as mean ± SEM. Scale bars: (A) 100 μm, (C and D) 150 μm, and (E) 200 μm.

See also Figures S5 and S7.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Cell, 45(5), Zhang, Y.M., Zimmer, M.A., Guardia, T., Callahan, S.J., Mondal, C., Di Martino, J., Takagi, T., Fennell, M., Garippa, R., Campbell, N.R., Bravo-Cordero, J.J., White, R.M., Distant Insulin Signaling Regulates Vertebrate Pigmentation through the Sheddase Bace2, 580-594.e7, Copyright (2018) with permission from Elsevier. Full text @ Dev. Cell