FIGURE

Fig. S1

ID

ZDB-FIG-180828-23

Publication

Gauvrit et al., 2018 - HHEX is a transcriptional regulator of the VEGFC/FLT4/PROX1 signaling axis during vascular development

Other Figures

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Fig. S1

zebrafish hhex mutants exhibit a delay during PHBC formation but form arterial intersegmental vessels

(a-b) Maximum intensity projections of confocal images of 24 hpf Tg(fli:nEGFP); hhex^+/+ and hhex^-/- embryos after GFP immunostaining. hhex mutants exhibit delayed primordial hindbrain channels (PHBCs) formation (arrowheads). (c-d) Maximum intensity projections of confocal images of 48 hpf TgBAC(etv2:EGFP); hhex^+/+ and hhex^-/- embryos. hhex mutants form arterial ISVs. (e-h) Trunk vasculature of 5 dpf Tg(kdrl:EGFP); Tg(-5.2lyve1b:DsRed); hhex^+/+ and hhex^-/-. hhex mutants form arterial ISVs while vessels from the PCV (vISVs and TD) are mostly absent (arrowheads point to the ventrally positioned TD; asterisks indicate lack of this structure). i) Quantification of vISVs across 10 somites in 5 dpf hhex^+/? (n=8) and hhex^-/- (n=8). Values represent means ± s.e.m. ****P ≤0.0001 by t-test. Scale bars: 100 μm (a-b), 200 μm (c-d), 50 μm (e-h).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	hhex^s721/s721; ci1Tg hhex^s721/s721; nz101Tg; s843Tg hhex^s721/s721; y7Tg hhex^s722/s722; ci1Tg hhex^s722/s722; nz101Tg; s843Tg hhex^s722/s722; y7Tg
Observed In:	intersegmental artery intersegmental vein lymph vessel endothelium posterior cardinal vein sprouting angiogenesis primordial hindbrain channel somite intersegmental vein thoracic duct intersegmental vessel
Stage Range:	Prim-5 to Day 5

Phenotype Detail

Acknowledgments

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