FIGURE

Fig. 5

ID

ZDB-FIG-180806-9

Publication

Rothschild et al., 2018 - Calcium Signals Act Through Histone Deacetylase to Mediate Pronephric Kidney Morphogenesis

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Fig. 5

CaMK‐II and HDACs act in the same molecular pathway leading to anterior kidney development. Embryos injected with the camk2g1 MO (1 ng), hdac 4 MO (2 ng), hdac5 MO (2 ng), camk2g1 and hdac MOs together, or the camk2g1 MO followed by bud‐stage trichostatin A treatment were imaged at 72 hpf from lateral perspectives using DIC optics (A–E) or dorsal perspectives using immunofluorescence of α1 Na⁺/K⁺ATPase (F–O). Scale bars = 100 μm. Arrowheads in I and N indicate adventitious lumens, and arrows indicate branched ducts. Convolution at 72 hpf (P) and pronephric migration (Q) as assessed by posterior ear‐anterior kidney distance in μm at 72 hpf. n = 30–41 embryos.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-camk2g1 WT + MO1-camk2g1 + MO4-hdac5 WT + MO1-camk2g1 + MO6-hdac4
Condition:	chemical treatment by environment: trichostatin A
Knockdown Reagents:	MO1-camk2g1 MO4-hdac5 MO6-hdac4
Observed In:	pronephros development pronephros morphogenesis
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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