FIGURE

Fig. 2

ID

ZDB-FIG-180806-6

Publication

Rothschild et al., 2018 - Calcium Signals Act Through Histone Deacetylase to Mediate Pronephric Kidney Morphogenesis

Other Figures

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Fig. 2

Kidney cysts form in hdac5 morphants. A–D: Dorsal images of embryos injected with 2 ng control, 2 ng HDAC4, 2 ng HDAC5, or 8 ng HDAC6 MO. Cysts were visible in hdac5 morphants (small arrows and inset, magnified view) at 48 hpf. Morphant embryos at 72 hpf were immunostained for α1 Na⁺K⁺ ATPase and imaged at low magnification (E–H) and high magnification (I–L). Scale bar = 100 μm. Anterior migration (convolution) is blocked in hdac5 morphants as summarized across the population by image analysis (M) and by the increase in distance between the anterior kidney and posterior ear (N). n = 40–70 embryos per experiment. *P < 0.005.

Expression Data

Antibody:	Ab1-atp1a1
Fish:	WT WT + MO4-hdac5 WT + MO5-hdac6 WT + MO6-hdac4
Knockdown Reagents:	MO4-hdac5 MO5-hdac6 MO6-hdac4
Anatomical Term:	pronephros
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO4-hdac5
Knockdown Reagent:	MO4-hdac5
Observed In:	pronephric glomerulus anatomical region pronephros development pronephros morphogenesis
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

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