Fig. S4
Azumolene treatment inhibits calcium mobilization and fin development, but not trunk neural crest migration. Related to Figure 4. (A) Traces of GCaMP fluorescence in electrically stimulated muscle of 1 day Tg(act2b:GCaMP6f) embryos. Caudal tips of tails were transected at 1 day and embryos were incubated in indicated solutions for 30 minutes at which time muscle was re-stimulated. Before cut (purple) indicates embryos prior to tail transection and drug treatment. Data are represented as mean ±SEM. The maximum change in fluorescence from baseline (arrow in A) is shown for each recorded embryo in (A’) with horizontal lines representing means. *p<0.01; ***p<0.0001; n.s., not significant. (B and C) Streams of migrating neural crest in the trunk, revealed by whole-mount in situ hybridization to detect crestin RNA expression, appear normal in ryr1a,3 morphants as compared to Tu controls. (D-G) RyR function is necessary for the Shh-dependent formation of pectoral fins. (D) Schematic dorsal view of 72hpf zebrafish embryo with region of interest outlined in box. Proximal/distal axis is indicated by blue arrow. (E-G) Images of fin buds in 72hpf embryos treated from 24 to 48hpf with indicated pharmacological agents. |
Reprinted from Developmental Cell, 45(4), Klatt Shaw, D., Gunther, D., Jurynec, M.J., Chagovetz, A.A., Ritchie, E., Grunwald, D.J., Intracellular Calcium Mobilization Is Required for Sonic Hedgehog Signaling, 512-525.e5, Copyright (2018) with permission from Elsevier. Full text @ Dev. Cell