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Fig. S4
Azumolene treatment inhibits calcium mobilization and fin development, but not trunk neural crest migration. Related to Figure 4. (A) Traces of GCaMP fluorescence in electrically stimulated muscle of 1 day Tg(act2b:GCaMP6f) embryos. Caudal tips of tails were transected at 1 day and embryos were incubated in indicated solutions for 30 minutes at which time muscle was re-stimulated. Before cut (purple) indicates embryos prior to tail transection and drug treatment. Data are represented as mean ±SEM. The maximum change in fluorescence from baseline (arrow in A) is shown for each recorded embryo in (A’) with horizontal lines representing means. *p<0.01; ***p<0.0001; n.s., not significant. (B and C) Streams of migrating neural crest in the trunk, revealed by whole-mount in situ hybridization to detect crestin RNA expression, appear normal in ryr1a,3 morphants as compared to Tu controls. (D-G) RyR function is necessary for the Shh-dependent formation of pectoral fins. (D) Schematic dorsal view of 72hpf zebrafish embryo with region of interest outlined in box. Proximal/distal axis is indicated by blue arrow. (E-G) Images of fin buds in 72hpf embryos treated from 24 to 48hpf with indicated pharmacological agents.
Reprinted from Developmental Cell, 45(4), Klatt Shaw, D., Gunther, D., Jurynec, M.J., Chagovetz, A.A., Ritchie, E., Grunwald, D.J., Intracellular Calcium Mobilization Is Required for Sonic Hedgehog Signaling, 512-525.e5, Copyright (2018) with permission from Elsevier. Full text @ Dev. Cell