FIGURE

Fig. 3

ID

ZDB-FIG-180620-48

Publication

Cai et al., 2018 - Knockout of zebrafish interleukin 7 receptor (IL7R) by the CRISPR/Cas9 system delays retinal neurodevelopment

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Fig. 3

The validation of the il7r^−/− mutant by western blotting and whole-mount in situ hybridisation.

a The expression of IL7R protein in WT and il7r^−/− larvae at 4 dpf. Note that no IL7R protein is detected in il7r^−/− larvae. b The images of whole-mount in situ hybridisation with gh1 (arrowheads) and rag1 (arrows) mRNA probes in WT and il7r^−/− larvae at 4 dpf. c-d The statistical analysis of gh1-positive area (c) or rag1-positive area (d) between WT and il7r^−/− larvae. Note that rag1 expression is significantly decreased in il7r^−/− larvae. Results are represented as means ± SEM, ***P < 0.001. The upper four panels in b: dorsal view. The lower two panels in b: dorsal is up, and rostral is left. Scale bar in b: 100 μm

Expression Data

Genes:	gh1 rag1
Fish:	TU il7r^nku2/nku2
Anatomical Terms:	hypophysis thymus
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	il7r^nku2/nku2
Observed In:	eye thymus thymus development whole organism
Stage Range:	Protruding-mouth to Day 4

Phenotype Detail

Acknowledgments

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