FIGURE

Fig. 4

ID

ZDB-FIG-180502-4

Publication

Li et al., 2017 - Axonemal dynein assembly requires the R2TP complex component Pontin

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Fig. 4

Pontin is essential for cilia motility. (A,B) Kymographs showing the rhythmic beating of motile cilia bundles in the pronephric tubule region (A) and olfactory placode (B) in wild-type zebrafish embryos at 3 dpf. pontin^{hi1055B/hi1055B} mutants (MUT) lack cilia motility. 10 wild-type and 9 pontin^{hi1055B/hi1055B} mutant embryos were observed. (C) Transmission electron micrographs of transverse sections of cilia in the pronephric tubule in wild type and pontin^{hi1055B/hi1055B} mutants at 5 dpf. Black arrowhead, outer dynein arm (ODA); red arrowhead, inner dynein arm (IDA). (D) Reduced number of ODAs, IDAs and total dynein arms per cilium section in pontin^{hi1055B/hi1055B} mutants in comparison with wild-type siblings. n=25. **P<0.01.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ruvbl1^{hi1055bTg/hi1055bTg} (AB/TU)
Observed In:	peripheral olfactory organ motile cilium pronephric tubule inner dynein arm pronephric tubule motile cilium pronephric tubule outer dynein arm
Stage Range:	Protruding-mouth to Day 5

Phenotype Detail

Acknowledgments

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