FIGURE

Fig. 2

ID

ZDB-FIG-180502-2

Publication

Li et al., 2017 - Axonemal dynein assembly requires the R2TP complex component Pontin

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Pontin and Reptin function together in zebrafish development. (A) pontin^{hi1055B/hi1055B} (pon), reptin^{hi2394/hi2394} (rep) and double mutants at 4 dpf show ventral body curvature and kidney cysts (arrows), as compared with a wild-type embryo. A total of 247 embryos were inspected. (B) Pontin and Reptin depend on each other for protein stability. Total lysates of reptin^{hi2394/hi2394} (rep, MUT), pontin^{hi1055B/hi1055B} (pon, MUT) and respective control siblings (C) at 4 dpf were subject to IP and blotted with anti-Reptin (Rep) or anti-Pontin (Pon). Western blot of β-Tubulin (Tub) on total lysates was used as a loading control. 20 embryos were used for each sample. The results shown are representative of two independent experiments.

Expression Data

Genes:	ruvbl1 ruvbl2
Antibodies:	Ab1-ruvbl1 Ab1-ruvbl2
Fish:	AB/TU ruvbl1^{hi1055bTg/hi1055bTg} (AB/TU) ruvbl2^{hi2394Tg/hi2394Tg} (AB/TU)
Anatomical Term:	whole organism
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ruvbl1^{hi1055bTg/hi1055bTg} (AB/TU) ruvbl2^{hi2394Tg/hi2394Tg} (AB/TU) ruvbl1^{hi1055bTg/hi1055bTg}; ruvbl2^{hi2394Tg/hi2394Tg} (AB/TU)
Observed In:	post-vent region pronephros trunk whole organism
Stage:	Day 4

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development