FIGURE

Fig. s5

ID

ZDB-FIG-180323-21

Publication

Herbert et al., 2017 - Dynein/dynactin is necessary for anterograde transport of Mbp mRNA in oligodendrocytes and for myelination in vivo.

Other Figures

All Figure Page

Back to All Figure Page

Fig. s5

Effects of ciliobrevin D treatment on zebrafish and cultured oligodendrocytes (related to Fig. 7). (A and B) DMSO and ciliobrevin D-treated fish at 4.5 dpf. Compared with fish treated with DMSO for 21 h (A), ciliobrevin D-treated fish look relatively normal. (Magnification: A and B, 16×.) (C) Treatment of oligodendrocytes differentiated for 3 d in vitro with different concentrations of the dynein inhibitor ciliobrevin for 21 h. Cell death was assayed by morphology using DIC microscopy; living cells contained continuous, nonbulbous branches and adhered to coverslips. Concentrations of 1 μM and 5 μM did not significantly alter the percentage of living cells, while 10 μM and 15 μM significantly decreased the percentage of living cells (n = 4 coverslips per group; *P = 0.023, ***P = 0.0001, one-way ANOVA).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA