FIGURE

Fig. 3

ID

ZDB-FIG-180323-13

Publication

Herbert et al., 2017 - Dynein/dynactin is necessary for anterograde transport of Mbp mRNA in oligodendrocytes and for myelination in vivo.

Other Figures

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Fig. 3

actr10^nl15/nl15 mutants have fewer OPCs. (A and B) Double transgenic tg(sox10:mgfp);tg(olig2:dsred) larvae were used to identify OPCs in spinal cord cross-sections at 3 dpf. Arrowheads mark OPCs, which are labeled by cytoplasmic DsRed and membrane-tagged GFP. (C) Mutants (n = 7) have significantly fewer OPCs in the spinal cord compared with sibling controls (n = 16; ***P < 0.0002). (D–G) The tg(olig2:dsred) line was used to count the number of olig2-labeled elongated cells in control animals (D and D′, arrows) compared with actr10^nl15/nl15 mutants (E and E′, arrows) at 3 dpf and at 4 dpf (pictured). (F) Quantification revealed a significant difference between the number of olig2-labeled dorsal cells in controls (n = 48) compared with actr10^nl15/nl15 mutants (n = 16; **P < 0.007) at 3 dpf. (G) Similarly, there was also a significant difference between controls (n = 28) and actr10^nl15/nl15 mutants (n = 16; ****P < 0.0001) at 4 dpf. Unpaired t tests with Welch’s correction were used for statistical analysis.

Expression Data

Genes:	DsRed2 EGFP
Fish:	vu19Tg sl3Tg; vu19Tg actr10^nl15/nl15; sl3Tg; vu19Tg actr10^nl15/nl15; vu19Tg
Anatomical Terms:	spinal cord glioblast (sensu Vertebrata) spinal cord motor neuron
Stage Range:	Protruding-mouth to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	actr10^nl15/nl15; sl3Tg; vu19Tg actr10^nl15/nl15; vu19Tg
Observed In:	spinal cord spinal cord glioblast
Stage Range:	Protruding-mouth to Day 4

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA