FIGURE

Fig. s4

ID

ZDB-FIG-180323-19

Publication

Herbert et al., 2017 - Dynein/dynactin is necessary for anterograde transport of Mbp mRNA in oligodendrocytes and for myelination in vivo.

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Fig. s4

Coimmunoprecipitation pulls out p135 in addition to p150^Glued (related to Figs. 6 and 7). (A) Image of a cell expressing a control construct for MS2-GFP, which contains a nuclear localization sequence (NLS). In the absence of any stem loop containing constructs, such as MBP, MS2-GFP localizes to the nucleus. (B) Identity of the p135 band from an anti-GFP immunoprecipitation against MS2-GFP–associated MBP mRNA granules was confirmed by comparing it to the eluted band from an immunoprecipitation using an antibody targeted against the N terminus of p150^Glued, which contains its microtubule-binding domain. (C) Ribbon diagram of DCTN1 isoform 1 (p150^Glued) shows a CAP-Gly microtubule-binding domain, which is lacking in DCTN1 isoform 2 (p135). (D) Kymograph analysis shows rapid back-and-forth movement of Mbp mRNA granules.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA