FIGURE

Fig. 4

ID

ZDB-FIG-180320-16

Publication

Kim et al., 2017 - Ottogi Inhibits Wnt/β-catenin Signaling by Regulating Cell Membrane Trafficking of Frizzled8

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

Loss of otg function affects AP-DV patterning and nuclear β-catenin localization. (A–C) Doral views, anterior to the left. Expression patterns of an anterior neural marker six3 and a posterior neural marker hoxa1 in control (A, 12/12, 100%), OTG mRNA-injected (B, 17/20, 85%) or otg MO-injected (C, 16/18, 89%) embryo at tail bud stage (10 hpf). (D–F) Doral views, posterior to the right. Expression pattern of a posterior mesoderm marker spt in control (D, 10/10, 100%), OTG mRNA-injected (E, 18/20, 90%) and otg MO-injected (F, 20/23, 87%) embryo at 10 somite stage. (G–J) Views of animal pole cells at blastula stage. Immunostaining for β-catenin and fluorescence intensity profile along the white lines in control (G), wnt8 mRNA-injected (H), wnt8 and OTG mRNA-injected (I), or wnt8 mRNA and otg MO-injected (J) embryo.

Expression Data

Genes:	hoxb1b six3b tbx16
Fish:	WT WT + MO1-prr7
Knockdown Reagent:	MO1-prr7
Anatomical Terms:	neuroectoderm anterior region neuroectoderm posterior region segmental plate
Stage Range:	Bud to 10-13 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-prr7
Knockdown Reagent:	MO1-prr7
Observed In:	neuroectoderm anterior region neuroectoderm posterior region segmental plate
Stage Range:	Bud to 10-13 somites

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.