FIGURE

Fig. S1

ID

ZDB-FIG-180221-25

Publication

Piatek et al., 2017 - Ectopically expressed Slc34a2a sense-antisense transcripts cause a cerebellar phenotype in zebrafish embryos depending on RNA complementarity and Dicer

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Fig. S1

Knockdown of p53 alleviates morpholino toxicity at high concentrations.

Non-target specific zebrafish embryo phenotypes can be caused by morpholino toxicity through the activation of p53 mediated apoptosis [59]. Therefore, a p53 morpholino was co-injected with the Dicer UTR morpholino to mitigate toxicity. Co-injection of 4 ng of p53 morpholino caused a partial rescue of 10 ng Dicer UTR injected samples. The rescue was only visible with high concentrations of Dicer UTR morpholino. When 4 ng p53 morpholino were co-injected with 2.5 ng Dicer UTR morpholino, 98.2% of embryos were classified as level 3. The predominant phenotypic feature were shorter body length and non-circular eyes.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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