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Fig. S1
Knockdown of p53 alleviates morpholino toxicity at high concentrations.
Non-target specific zebrafish embryo phenotypes can be caused by morpholino toxicity through the activation of p53 mediated apoptosis [59]. Therefore, a p53 morpholino was co-injected with the Dicer UTR morpholino to mitigate toxicity. Co-injection of 4 ng of p53 morpholino caused a partial rescue of 10 ng Dicer UTR injected samples. The rescue was only visible with high concentrations of Dicer UTR morpholino. When 4 ng p53 morpholino were co-injected with 2.5 ng Dicer UTR morpholino, 98.2% of embryos were classified as level 3. The predominant phenotypic feature were shorter body length and non-circular eyes.