FIGURE

Fig. 5

ID

ZDB-FIG-180118-33

Publication

Lin et al., 2017 - Klf8 regulates left-right asymmetric patterning through modulation of Kupffer's vesicle morphogenesis and spaw expression

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Fig. 5

Knockdown of klf8 affected dorsal forerunner cell number, KV cilia number and length. Cell number of dorsal forerunner cells (DFCs) was affected in klf8-MO1^atg- or klf8-MO2^atg -injected embryos as compared to klf8-4 mm MO1-injected Tg(sox17:gfp) control embryos (4 mm) at 75% epiboly (a-d). Images of KV cilia stained with acetylated tubulin antibody in klf8 morphants (g-i) and control embryos (e, f) at 10s stage (e-i). Images of acetylated tubulin stained KV cilia and GFP stained DFCs in Tg(sox17:gfp) embryos injected with different klf8 MOs (l-n), klf8-4 mm MO1 (k) or wild type (j) embryos at 10s stage (j-n). DFC number (o), KV cilia number (q), length (r) but not lumen area (p) were affected in klf8 morphants as compared to control embryos. Statistical significance was determined by Student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001. Error bars indicate standard deviation

Expression Data

Gene:	GFP
Antibody:	Ab1-tuba
Fish:	s870Tg s870Tg + MO1-klf8 s870Tg + MO2-klf8 s870Tg + MO3-klf8 + MO4-klf8 + MO5-klf8
Knockdown Reagents:	MO1-klf8 MO2-klf8 MO3-klf8 MO4-klf8 MO5-klf8
Anatomical Terms:	forerunner cell group Kupffer's vesicle Kupffer's vesicle cilium
Stage Range:	75%-epiboly to 10-13 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	s870Tg + MO1-klf8 s870Tg + MO2-klf8 s870Tg + MO3-klf8 + MO4-klf8 + MO5-klf8
Knockdown Reagents:	MO1-klf8 MO2-klf8 MO3-klf8 MO4-klf8 MO5-klf8
Observed In:	forerunner cell group Kupffer's vesicle cilium whole organism
Stage Range:	75%-epiboly to 10-13 somites

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Biomed. Sci.