FIGURE

Fig. 1

ID

ZDB-FIG-180104-18

Publication

Sandoval et al., 2017 - A metabolic switch controls intestinal differentiation downstream of Adenomatous polyposis coli (APC).

Other Figures

All Figure Page

Back to All Figure Page

Fig. 1

mpc1 and mpc2 are downregulated in apc^mcr and apc morphant embryos.

(A,B) Quantitative RT-PCR analysis of mpc1 and mpc2 gene expression in apc^mcr (A) and apc mo (B) embryos. Values represent mean ± SD. Graph shown above is representative of at least three independent experiments. Statistical significance was analyzed using unpaired t-test. (C,D) Whole mount in situ hybridization for mpc1 and mpc2 in 72 hpf apc^mcr (C) and apc mo (D) embryos. (E) Whole mount in situ hybridization for mpc1 and mpc2 in wild type (WT) embryos. head (h), eyes (e), somite (som), vasculature (vas), gut (g), liver (l). (F) Cross sections from 96 hpf WT embryos probed with either mpc1 or mpc2 confirmed gut-specific expression of both genes. See also Figure 1—figure supplement 1.

Expression Data

Genes:	mpc1 mpc2b
Fish:	TU apc^hu745/hu745 TU + MO3-apc
Knockdown Reagent:	MO3-apc
Anatomical Terms:	eye gut head liver pectoral fin somite trunk vasculature whole organism
Stage Range:	Prim-5 to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	apc^hu745/hu745 TU + MO3-apc
Knockdown Reagent:	MO3-apc
Observed In:	gut head trunk whole organism
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife