FIGURE

Fig. 4

ID

ZDB-FIG-171206-48

Publication

Chang et al., 2017 - Ftr82 Is Critical for Vascular Patterning during Zebrafish Development

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Fig. 4

Knockdown of ftr82 can be rescued by overexpression of ftr82. In uninjected control embryos, ISV has reached the dorsal region and formed DLAV around 28–30 hpf ((A), arrowheads). At the same stage in fr82 MO, ISVs have stalled or slowed growth at mid-somite ((C), hollow arrowheads). Overexpression of ftr82 by ftr82 mRNA injection caused no obvious defect in vasculature (B), but rescued the defect of ISV stalling ((D), solid arrowheads); (E) Quantification of the percentage of completed ISV at 30 hpf shows a ~45% increase in rescued embryos compared to ftr82 morphants. Percentages of completed ISV are ~97, 22 ± 12, 92 ± 8, and 67 ± 7 in control, ftr82 MO, ftr82 mRNA overexpression, and rescued embryos, respectively. *** refers to p < 0.0001 by an unpaired Student’s t-test. Data represent means ± S.D. Scale bars are 100 μm for (A–D).

Expression Data

Gene:	EGFP
Fish:	y1Tg y1Tg + MO2-ftr82
Knockdown Reagent:	MO2-ftr82
Anatomical Term:	vasculature
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	y1Tg + MO2-ftr82
Knockdown Reagent:	MO2-ftr82
Observed In:	intersegmental vessel
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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