Fig. S1

Knockdown of ftr82 causes defects in vascular development in zebrafish by using translational block ATG morpholino. (A–F) Bright-field images show the morphology of uninjected control Tg(flk:gfp) embryos (control, A) and ftr82 translational block ATG morpholino injection (ftr82^atg MO) at 26 hpf (B); (C–F) Loss of ftr82 shows intersegmental vessel (ISV) growth defect (hollow arrowheads in (D)) compared to control (arrowheads in (C)), and less angiogenic sprouting from the tail caudal vein in ftr82 morphants (arrows in E,F) at 26 hours post-fertilization (hpf). (E) and (F) are enlarged figures from (C) and (D), respectively; (G–L) At 30 hpf, in uninjected control embryos, ISV have reached the dorsal longitudinal anastomotic vessel (DLAV) at the dorsal part of the embryo (G, arrowheads) and the caudal vein plexus (CVP) formed loop structures at the tail (I, arrows). Knockdown of ftr82 at the same stage showed ISVs growth stall in the middle line of somite (H, hollow arrowheads) and less honeycomb structure in the CVP (J, arrows); Reduced number of cells per ISV was photographed and counted in ftr82 morphant embryos (2.0 ± 0.5, n = 25) versus in control Tg(fli1a:negfp)^y7 (3.8 ± 0.4, n = 22) (K,L,Q). At 48 hpf, less or no capillaries loop in the CVP was observed (N) compared to control (arrows in M); (O) Quantification of angiogenic sprouting from the caudal vein shows a 4.5-fold decreased in ftr82 morphants at 26 hpf (n = 23 in control and in n = 22 in ftr82 MO); (P) Quantification of percentage of completed ISV in ftr82 morphants shows a ~40% decrease (n = 24 in control and n = 26 in ftr82^atg MO) at 30 hpf. *** refers to p < 0.0001 by an unpaired Student's t-test. Scale bars are 200 μm for A–D, 100 μm for E–N.