FIGURE

Fig. S5

ID

ZDB-FIG-171117-8

Publication

Lee et al., 2016 - Myc and Fgf Are Required for Zebrafish Neuromast Hair Cell Regeneration

Other Figures

All Figure Page

Back to All Figure Page

Fig. S5

The Myc inhibitor does not induce apoptosis.

5-dpf zebrafish larvae with neomycin treatment were then treated with or without 100 nM c-MYC inhibitor Int-H1-S6A, F8A for 72 hrs (G-I, J-L). Larvae without neomycin and inhibitor treatment (No Trt) and larvae collected 1 hr after neomycin treatment were used as controls. The fish were stained with HCS1 antibody (A,D,G,J) to label HCs and TUNEL assay (B,E,H,K) to measure apoptosis. No significant difference in apoptosis signal was observed between inhibitor-treated and non-treated fish (TUNEL⁺ cells per neuromast: 0.4 ± 0.2 for No Trt, n = 14; 0.4 ± 0.1 for Neo 72hr, n = 15; 0.6 ± 0.2 for Neo/Myc-pep 72hr, n = 15). All TUNEL signals were from outside of the neuromast (I,L). In the positive control (D-F), a significant increase in the TUNEL⁺ cells were seen inside the neuromast. Scale bars: 10 μm

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One