FIGURE

Fig. 4

ID

ZDB-FIG-171030-12

Publication

Kotani et al., 2015 - Neuromuscular regulation in zebrafish by a large AAA+ ATPase/ubiquitin ligase, mysterin/RNF213

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Fig. 4

Fast muscle-specific expression of human mysterin restores the fast muscle malformation in morphants.

(a) Ectopic expression of human mysterin restores the morphology of fast muscle in mysterin morphant larvae. Labeling of F-actin shows the formation of robust muscle fibers upon ectopic expression of mysterin in fast muscle, but not in the control. Expression of RFP driven by the UAS promoter shows that these larvae carry the GAL4FF-expressing transgene in fast muscle. (b) EM observation of restored fast muscle in the trunk. The lower panels show magnified images. (c) Projection of primary motoneurons in somites. Projection of motoneurons is impaired in mysterin-α morphants and restored by fast muscle-specific expression of human mysterin (white arrows). (d) Effect of suppression of mysterin-α and fast muscle-specific expression of human mysterin on vascular formation. Human mysterin tagged with a 3×FLAG epitope (WT-3×FLAG) is expressed in transgenic zebrafish that express GFP in vascular endothelial cells. Green (GFP) and magenta (F-actin) show trunk vessels and fast muscle, respectively.

Expression Data

Gene:	RFP
Antibody:	znp-1
Fish:	nkgSA2AzGFF598AGt; nkuasrfp1aTg nkgSA2AzGFF598AGt; nkuasrfp1aTg + MO2-rnf213a
Knockdown Reagent:	MO2-rnf213a
Anatomical Terms:	CaP motoneuron axon fast muscle cell MiP motor neuron axon RoP motor neuron axon
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nkgSA2AzGFF598AGt; nkuasrfp1aTg + MO2-rnf213a
Knockdown Reagent:	MO2-rnf213a
Observed In:	CaP motoneuron axon extension involved in axon guidance fast muscle cell fast muscle cell skeletal muscle fiber development fast muscle cell skeletal muscle myofibril fast muscle cell Z disc MiP motor neuron axon extension involved in axon guidance myotome fast muscle cell RoP motor neuron axon extension involved in axon guidance
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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