FIGURE

Fig. 2

ID

ZDB-FIG-171030-11

Publication

Kotani et al., 2015 - Neuromuscular regulation in zebrafish by a large AAA+ ATPase/ubiquitin ligase, mysterin/RNF213

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Fig. 2

Suppression of mysterin-α leads to impaired projection of primary motoneurons during early development.

(a) Schematic representation of motoneuron projection in somites during the early development of zebrafish. Primary CaP (orange), MiP (purple), and RoP (green) motoneurons project axons from the spinal code (sc). Each primary motoneuron projects its axon to a different area of muscle. In particular, intersegmental axonal projection of MiP and CaP motoneurons is indicated by blue arrows. The notochord is indicated by nc. (b) Motoneuron projection in the trunk region of a mysterin-α-suppressed animal (high dose). Primary motoneurons at 2 dpf are stained with an anti-synaptotagmin antibody (znp-1). The control animal exhibits proper projection of CaP and MiP motoneurons along the myotome segment and branched axons into intersegmental areas (white arrows). The morphant exhibits immature projection of CaP and MiP motoneurons along the myotome segment and reduced branched axons (red arrows).

Expression Data

Antibody:	znp-1
Fish:	WT WT + MO2-rnf213a
Knockdown Reagent:	MO2-rnf213a
Anatomical Terms:	CaP motoneuron axon MiP motor neuron axon RoP motor neuron axon
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-rnf213a
Knockdown Reagent:	MO2-rnf213a
Observed In:	CaP motoneuron axon extension involved in axon guidance MiP motor neuron axon extension involved in axon guidance RoP motor neuron axon extension involved in axon guidance
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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