FIGURE

Fig. 6

ID

ZDB-FIG-170922-41

Publication

Liu et al., 2017 - Zebrafish B Cell Development without a Pre-B Cell Stage, Revealed by CD79 Fluorescence Reporter Transgenes

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Fig. 6

Zebrafish B cell response to bacterial infection with CD79 reduction. (A) Low-power images of intestinal region of PBS control and bacteria injected CD79a-GFP fish at 1 wk postinfection. Scale bars, 1 mm. (B) Percentage of CD79a⁺⁺ and CD79a⁺ cells in indicated tissues at 1, 2, and 4 wk postinfection; n = 3 each, mean ± SE. (C) Flow cytometry histograms of CD79a⁺⁺ and CD79a⁺ cells in kidney and intestine tissue 1 wk postinfection, showing the clear increase in low CD79⁺ cells in intestine. (D) Increase in size of CD79a⁺ cells from intestine of infected fish, detected by flow cytometry using forward light scatter (FSC). (E) CD79a⁺ cells are enriched for secreted IgM compared with CD79a⁺⁺ cells. RT-PCR analysis of β-actin, membrane IgH-μ (m), and secreted IgH-μ (s).

Expression Data

Gene:	EGFP
Fish:	fcc89Tg
Condition:	bacterial treatment by injection: Pseudomonas aeruginosa PA14
Anatomical Terms:	head kidney intestine liver spleen
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	fcc88Tg fcc89Tg
Condition:	bacterial treatment by injection: Pseudomonas aeruginosa PA14
Observed In:	B cell intestine liver spleen
Stage:	Adult

Phenotype Detail

Acknowledgments

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