FIGURE

Fig. 4

ID

ZDB-FIG-170922-40

Publication

Liu et al., 2017 - Zebrafish B Cell Development without a Pre-B Cell Stage, Revealed by CD79 Fluorescence Reporter Transgenes

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Fig. 4

Identification of developing and mature B cells in CD79a-GFP and CD79b-GFP × Rag2-mCherry double-transgenic zebrafish. (A) B cells in young CD79a-GFP and CD79b-GFP zebrafish larvae identified by coexpression with Rag2:mCherry, analyzed by flow cytometry. Wild-type zebrafish AB line is shown as a control. Representative data of four to seven sample analyses from each day postferilization zebrafish are shown. (B) Kidney marrow of 1–8 mo CD79a-GFP × Rag2-mCherry. Dotted region is GFP⁻mCherry⁻. (C) Five month CD79b-GFP × Rag2-mCherry zebrafish. Low-power (top) and high-power (bottom) images of thin sections of kidney and thymus are shown. Scale bars, 200 μm (top) and 100 μm (bottom). (D) Flow cytometry analysis of adult CD79b-GFP × Rag2-mCherry zebrafish kidney and thymus. Dotted region is GFP⁻mCherry⁻. Frequency of CD79b:GFP⁺ cells in kidney is 26.4%, in thymus is 5%. Data are representative of five separate CD79b/Rag2 transgenic fish kidney analyses.

Expression Data

Genes:	EGFP mCherry
Fish:	bu3Tg; fcc89Tg
Anatomical Terms:	head kidney thymus
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Immunol.