ZFIN Image: Liu et al., 2017, Fig. 6

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Figure Caption

Fig. 6

Zebrafish B cell response to bacterial infection with CD79 reduction. (A) Low-power images of intestinal region of PBS control and bacteria injected CD79a-GFP fish at 1 wk postinfection. Scale bars, 1 mm. (B) Percentage of CD79a⁺⁺ and CD79a⁺ cells in indicated tissues at 1, 2, and 4 wk postinfection; n = 3 each, mean ± SE. (C) Flow cytometry histograms of CD79a⁺⁺ and CD79a⁺ cells in kidney and intestine tissue 1 wk postinfection, showing the clear increase in low CD79⁺ cells in intestine. (D) Increase in size of CD79a⁺ cells from intestine of infected fish, detected by flow cytometry using forward light scatter (FSC). (E) CD79a⁺ cells are enriched for secreted IgM compared with CD79a⁺⁺ cells. RT-PCR analysis of β-actin, membrane IgH-μ (m), and secreted IgH-μ (s).

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Immunol.