Surviving hepatocytes become hybrid hepatocytes. (A) Confocal single-optical section images showing the expression of Hnf4a (green), Tp1:H2B-mCherry (red), and Bhmt (gray) in the liver. Arrows point to hepatocytes that express Tp1:H2B-mCherry; arrowheads point to BECs negative for Hnf4a and Bhmt. (B) Confocal projection images showing Tp1:H2B-mCherry (red) and Anxa4 (gray) expression in the liver. Arrows point to H2B-mCherry/Anxa4 double-positive cells. (C) Confocal single-optical section images showing the hepatic expression of ubb:mCherry (red, Cre-labeled cells) and Hnf4a (gray) in tomm22 MO-injected larvae. Arrows point to mCherry/Hnf4a double-positive cells. (D) Graph showing the percentage of ubb:mCherry+ cells among Hnf4a+ cells, which were derived from BECs. Red dots indicate the larvae shown in (C); n indicates the number of larvae examined. Scale bars: 20 μm; error bars: ±SEM.
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