FIGURE

Fig. 4

ID

ZDB-FIG-170807-5

Publication

Wu et al., 2017 - tomm22 Knockdown-Mediated Hepatocyte Damages Elicit Both the Formation of Hybrid Hepatocytes and Biliary Conversion to Hepatocytes in Zebrafish Larvae.

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Fig. 4

Surviving hepatocytes become hybrid hepatocytes. (A) Confocal single-optical section images showing the expression of Hnf4a (green), Tp1:H2B-mCherry (red), and Bhmt (gray) in the liver. Arrows point to hepatocytes that express Tp1:H2B-mCherry; arrowheads point to BECs negative for Hnf4a and Bhmt. (B) Confocal projection images showing Tp1:H2B-mCherry (red) and Anxa4 (gray) expression in the liver. Arrows point to H2B-mCherry/Anxa4 double-positive cells. (C) Confocal single-optical section images showing the hepatic expression of ubb:mCherry (red, Cre-labeled cells) and Hnf4a (gray) in tomm22 MO-injected larvae. Arrows point to mCherry/Hnf4a double-positive cells. (D) Graph showing the percentage of ubb:mCherry⁺ cells among Hnf4a⁺ cells, which were derived from BECs. Red dots indicate the larvae shown in (C); n indicates the number of larvae examined. Scale bars: 20 μm; error bars: ±SEM.

Expression Data

Gene:	mCherry
Antibodies:	Ab-2F11 Ab1-bhmt
Fish:	s939Tg s939Tg + MO1-tomm22 cz1701Tg; s959Tg + MO1-tomm22
Condition:	chemical treatment: afimoxifene
Knockdown Reagent:	MO1-tomm22
Anatomical Terms:	cholangiocyte hepatocyte
Stage Range:	Day 4 to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	s939Tg + MO1-tomm22 cz1701Tg; s959Tg + MO1-tomm22
Condition:	chemical treatment: afimoxifene
Knockdown Reagent:	MO1-tomm22
Observed In:	hepatocyte
Stage Range:	Day 4 to Days 7-13

Phenotype Detail

Acknowledgments

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