FIGURE

Fig. 2

ID

ZDB-FIG-170807-3

Publication

Wu et al., 2017 - tomm22 Knockdown-Mediated Hepatocyte Damages Elicit Both the Formation of Hybrid Hepatocytes and Biliary Conversion to Hepatocytes in Zebrafish Larvae.

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Fig. 2

Cell death and proliferation in tomm22 MO-injected larvae. (A) Confocal projection images showing Prox1 expression (gray) and terminal deoxynucleotidyl transferase (TdT) dUTP nick-end labeling (TUNEL) (green) in the liver bud (dashed lines) at 36 h postfertilization (hpf). Arrow points to TUNEL⁺ cells. (B) Confocal projection images showing TUNEL (green) and the expression of fabp10a:CFP-NTR (blue) and Tp1:H2B-mCherry (red) in the liver at 4 dpf. Arrow points to TUNEL⁺ hepatocytes. (C) Confocal projection images showing Prox1 expression (green) and 5-ethynyl-2′-deoxyuridine (EdU) labeling (gray) in the liver (dashed lines) at 45 hpf. Arrows point to EdU/Prox1 double-positive cells. (D) Graph showing the percentage of EdU⁺ cells among Prox1⁺ cells in the liver at 45 hpf. There was no significant difference in the proliferation rate between the control and tomm22 MO-injected larvae. Red marks indicate the embryos shown in (C); n indicates the number of larvae examined. Scale bars: 20 μm; error bars: ±SEM.

Expression Data

Genes:	CFP mCherry
Fish:	s931Tg; s939Tg s931Tg; s939Tg + MO1-tomm22
Knockdown Reagent:	MO1-tomm22
Anatomical Terms:	cholangiocyte hepatocyte
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-tomm22 s931Tg; s939Tg + MO1-tomm22
Knockdown Reagent:	MO1-tomm22
Observed In:	liver cell death
Stage Range:	Prim-25 to Day 4

Phenotype Detail

Acknowledgments

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