FIGURE

Fig. 5

ID

ZDB-FIG-170807-25

Publication

Passoni et al., 2017 - Imaging of viral neuroinvasion in the zebrafish reveals that Sindbis and chikungunya viruses favour different entry routes

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Fig. 5

Macrophages are not infected by SINV or CHIKV and not required for neuroinvasion. (A,B) Whole-mount immunohistochemistry of SINV-infected (A) or CHIKV-infected (B) mpeg:G/U:Nfsb-mCherry (macrophages in magenta) larva at 2 dpi. Lateral views of the head; confocal imaging, maximal projection. (A′,B′) Merge of transmitted light with green (infected cells) and magenta (macrophages) fluorescence. (A″,B″) Green fluorescence showing infected cells. (A‴,B‴) Magenta fluorescence showing macrophage distribution. (A‴′,B‴′) Magnification of the area boxed in A′ and B′; green and magenta fluorescence, 3D rendering slightly tilted. Scale bars: 50 µm (A′-A‴,B′-B‴), 25 µm (A″″,B″″). (C) Macrophage depletion in mpeg:G/U:Nfsb-mCherry larvae. Superposition of transmitted light and magenta (macrophage) fluorescence, maximal projection, lateral view of the head, in a DMSO-treated control (top) or 2 days after treatment with metronidazole (bottom). (D) Impact of macrophage depletion on occurrence of neuroinvasion. mpeg:G/U:Nfsb-mCherry larvae were treated with DMSO or metronidazole, before injection with SINV (top graph) or CHIKV (bottom graph). Percentage of brain-infected larvae. n=36 from three independent experiments pooled. ns, not significant (Log-rank test).

Expression Data

Gene:	mCherry
Fish:	c264Tg; gl25Tg
Conditions:	chemical treatment by environment: metronidazole viral treatment by injection: Chikungunya virus viral treatment by injection: Sindbis virus
Anatomical Term:	macrophage
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	c264Tg; gl25Tg
Condition:	chemical treatment by environment: metronidazole
Observed In:	macrophage
Stage:	Day 5

Phenotype Detail

Acknowledgments

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