FIGURE

Fig. 4

ID

ZDB-FIG-170807-24

Publication

Passoni et al., 2017 - Imaging of viral neuroinvasion in the zebrafish reveals that Sindbis and chikungunya viruses favour different entry routes

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Fig. 4

SINV does not induce BBB leakage. In vivo assay for BBB permeability. Uninfected controls, IHNV- and SINV-infected fliG/U:RFP larvae were injected IV at 1 and 2 dpi with 10 kDa FITC-dextran. One hour later, live confocal imaging of the brain was performed, to compare FITC intensity within the CBV and BP. (Injections at 2 dpi were performed only on uninfected controls and SINV-infected larvae, because of early death of IHNV-infected larvae.) (A,B) Representative images of IHNV-infected (A) and SINV-infected (B) larvae; single confocal plane at same depth. Scale bars: 100 μm. (C) Ratios of FITC fluorescence intensities measured in BP and CBV. n=2-5 larvae per group; two separate focal planes per larva.

Expression Data

Gene:	RFP
Fish:	nkuasrfp1aTg; ubs4Tg
Conditions:	viral treatment by injection: Infectious hematopoietic necrosis virus viral treatment by injection: Sindbis virus
Anatomical Term:	endothelial cell
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nkuasrfp1aTg; ubs4Tg
Condition:	viral treatment by injection: Infectious hematopoietic necrosis virus
Observed In:	maintenance of blood-brain barrier
Stage:	Day 4

Phenotype Detail

Acknowledgments

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