FIGURE

Fig. 4

ID

ZDB-FIG-170502-3

Publication

Zhang et al., 2017 - Critical Role for GAB2 in Neuroblastoma Pathogenesis through the Promotion of SHP2/MYCN Cooperation

Other Figures

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Fig. 4

Mutant ptpn11 Enhances the Proliferation of MYCN-Induced Hyperplastic Neuroblasts in the IRG

(A) Sagittal sections through the IRGs of control Dβh, ptpn11mut, MYCN, and MYCN;ptpn11mut transgenic fish at 5 wpf (dorsal up, anterior left). GFP, green; Hu, red; DAPI, blue; merge, combined green, red, and blue. Representative sections through the IRG of control Dβh or ptpn11mut fish contain 1-5 GFP+/Hu+ sympathetic neuroblasts (arrowheads). Hu+ cell numbers increase in MYCN and MYCN;ptpn11mut fish (brackets). Dotted lines indicate the head kidney (HK) boundary. Scale bar, 20 μm.

(B) Numbers of GFP+/Hu+ sympathetic neuroblasts in the IRG regions of control Dβh, ptpn11mut, MYCN, and MYCN;ptpn11mut transgenic fish at 5 wpf.

(C) Percentage of EdU+/Hu+ sympathetic neuroblasts in control Dβh, ptpn11mut, MYCN, and MYCN;ptpn11mut transgenic fish at 5 weeks. Mean values (horizontal bars) were compared by Welch t test (two-tailed).

See also Figures S3 and S4.

Expression Data

Gene:	EGFP
Antibody:	Ab1-elavl
Fish:	zdf22Tg (AB) zf738Tg (AB) zdf16Tg (AB) zdf16Tg; zf738Tg (AB)
Anatomical Terms:	interrenal gland neuroblast interrenal gland sympathetic neuron
Stage:	Days 30-44

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	zdf16Tg (AB) zdf16Tg; zf738Tg (AB)
Observed In:	interrenal gland neural precursor cell proliferation interrenal gland neuroblast
Stage:	Days 30-44

Phenotype Detail

Acknowledgments

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