FIGURE

Fig. 5

ID

ZDB-FIG-170203-15

Publication

Miao et al., 2017 - Translation repression by maternal RNA binding protein zar1 is essential for early oogenesis in zebrafish

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Fig. 5

EE2 treatment restores ovarian development in zar1 homozygous mutant. (A) Statistics for sex ratio of zar1 homozygous and heterozygous mutants after EE2 treatment. (B,C) H&E staining of ovarian tissue in EE2-treated zar1 homozygotes and heterozygotes. Similar to zar1 heterozygous ovaries, the treated homozygous mutant ovaries possess oocytes at all stages. (D) btg2 and traf4a expression analyzed by qPCR in zar1 homozygotes (n=6) and heterozygotes (n=6). (E) atf3 expression analyzed by qPCR in zar1 homozygotes (n=7) and heterozygotes (n=8) with or without EE2 treatment. Gene expression was normalized to expression of elongation factor 1 alpha (ef1a). hetero, zar1 heterozygotes; homo, zar1 homozygotes; EE2-hetero, EE2-treated zar1 heterozygotes (n=6); EE2-homo, EE2 treated zar1 homozygotes (n=6). Data are mean±s.e.m. **P<0.01, ***P<0.001; ns, not significant. Scale bar: 20 μm.

Expression Data

Genes:	atf3 btg2 traf4a
Fish:	zar1^gd5/gd5 (TU) zar1^gd5/+ (TU)
Condition:	chemical treatment by environment: 17alpha-ethynylestradiol
Anatomical Term:	ovary
Stage:	Days 30-44

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	zar1^gd5/gd5 (TU) zar1^gd5/+ (TU)
Condition:	chemical treatment by environment: 17alpha-ethynylestradiol
Observed In:	female organism female sex determination male organism ovary
Stage Range:	Days 30-44 to Adult

Phenotype Detail

Acknowledgments

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