FIGURE

Fig. 1

ID

ZDB-FIG-170203-11

Publication

Miao et al., 2017 - Translation repression by maternal RNA binding protein zar1 is essential for early oogenesis in zebrafish

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Fig. 1

Generation of a zar1 mutant with TALENs in zebrafish. (A) In situ hybridization on cryosections of wild-type gonads with zar1 antisense probe and sense probe. Scale bar: 200 μm. (B) Immunoblotting to detect Zar1 protein in stage I, stage II and stage III oocytes. Ten oocytes were lysed for each stage. (C) The TALEN sequences for zar1 mutant generation. (D) Digestion of PCR products from wild-type (WT) and zar1 TALEN mRNA injected embryos (TLN) with NdeI restriction enzyme. (E) DNA sequences of the zar1^gd5 mutant fish line. A premature stop codon was generated. (F) Western blot of Zar1 in gonads from WT, heterozygotes (hetero) and homozygotes (homo) at 25 dpf. DNA sequences highlighted in yellow are the start codon; red, NdeI recognition sites; green, premature stop codon.

Expression Data

Gene:	zar1
Antibody:	Ab1-zar1
Fish:	TU WT zar1^gd5/gd5 (TU) zar1^gd5/+ (TU)
Anatomical Terms:	gonad oocyte stage I oocyte stage II
Stage Range:	Days 21-29 to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	zar1^gd5/gd5 (TU)
Observed In:	gonad
Stage:	Days 21-29

Phenotype Detail

Acknowledgments

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