FIGURE

Fig. 7

ID

ZDB-FIG-170123-15

Publication

Miranda-Rodríguez et al., 2017 - RhoA/ROCK pathway activity is essential for the correct localization of the germ plasm mRNAs in zebrafish embryos

Other Figures

All Figure Page

Back to All Figure Page

Fig. 7

ROCK inhibition disturbs the localization of vasa and nanos mRNAs at the distal part of the cleavage furrow at 4-cells stage zebrafish embryos. nanos and vasa in situ hybridization localization in 4-cell stage embryos with the normal localization at the distal edge of the cleavage furrow (A, a1 and C, c1 respectively). h1152 Injected embryos showed abnormal localization of nanos (B and b1) and vasa (D and d1) at the center of the cleavage furrow. Quantification of normal localization of the mRNAs aggregates of nanos (E) or vasa (F), in embryos injected with control buffer or h1152. (E) Localization of nanos in control (n=160) and embryos injected with h1152 (n=209) showed statistical differences. (F) Localization of vasa in control (n=138) and h1152 treated embryos (n=131) also showed statistical differences from 4 independent experiments. The error bars represent the standard error of the mean (** p<0.01).

Expression Data

Genes:	ddx4 nanos3
Fish:	WT
Condition:	chemical treatment by injection: (S)-2-methyl-1-(4-methylisoquinoline-5-sulfonyl)-1,4-diazepane dihydrochloride
Anatomical Terms:	blastodisc cleavage furrow blastodisc germ plasm blastomere central region
Stage:	4-cell

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT
Condition:	chemical treatment by injection: (S)-2-methyl-1-(4-methylisoquinoline-5-sulfonyl)-1,4-diazepane dihydrochloride
Observed In:	blastodisc cleavage furrow blastomere central region
Stage:	4-cell

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 421(1), Miranda-Rodríguez, J.R., Salas-Vidal, E., Lomelí, H., Zurita, M., Schnabel, D., RhoA/ROCK pathway activity is essential for the correct localization of the germ plasm mRNAs in zebrafish embryos, 27-42, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.