FIGURE

Fig. 4

ID

ZDB-FIG-170104-11

Publication

Jiménez-Amilburu et al., 2016 - In Vivo Visualization of Cardiomyocyte Apicobasal Polarity Reveals Epithelial to Mesenchymal-like Transition during Cardiac Trabeculation

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Fig. 4

CM Depolarization Is Nrg/ErbB2 Signaling-, and Blood Flow- and Cardiac Contractility-Dependent

(A–B'') Confocal images (mid-sagittal sections) of 100-hpf Tg(−0.2myl7:EGFP-podxl);Tg(myl7:MKATE-CAAX) zebrafish hearts. Boxed area in (A) is shown in (A') and (A''). Untreated larvae show apical constrictions (A' and A'', arrowheads) and depolarized CMs (A' and A'', asterisks). (B–B''). Larvae treated with 10 μM PD168393 from 56 to 100 hpf exhibit no apical constrictions or depolarized CMs (B' and B'', arrows). Boxed areas in (B) are shown in (B') and (B'').

(C–D') Confocal images (mid-sagittal sections) of 82-hpf Tg(−0.2myl7:EGFP-podxl) zebrafish hearts from a nrg2a^+/− incross. Boxed areas in (C) and (D) are shown in (C') and (D'). Heterozygous larvae (nrg2a^+/−) exhibit an average of three apical constrictions per ventricle at the mid-sagittal plane (C and C′, arrowheads), while homozygous mutants (nrg2a^−/−) exhibit a reduced number of apical constrictions (D and D', arrowheads).

(E) Graph showing the number of apical constrictions in nrg2a heterozygous and homozygous mutant larvae; n = 4 and n = 6, respectively. Each dot represents one heart. Data are shown as mean ± SEM. ^∗∗∗p < 0.001 by Student’s t test.

(F–F'') 100-hpf tnnt2a morphant hearts exhibit no apical constrictions or depolarized CMs (F' and F'', arrows). Boxed areas in (F) are shown in (F') and (F'').

At, atrium; V, ventricle; AVC, atrioventricular canal. Scale bars, 20 μm.

Expression Data

Genes:	EGFP mKate2
Fish:	bns103Tg bns103Tg; sd11Tg bns103Tg; sd11Tg + MO1-tnnt2a nrg2a^{mn0237Gt/mn0237Gt}; bns103Tg
Condition:	chemical treatment: PD 168393
Knockdown Reagent:	MO1-tnnt2a
Anatomical Terms:	cardiac muscle cell apical plasma membrane cardiac muscle cell membrane
Stage Range:	Protruding-mouth to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	bns103Tg; sd11Tg nrg2a^{mn0237Gt/mn0237Gt}; bns103Tg
Condition:	chemical treatment: PD 168393
Observed In:	cardiac muscle cell cardiac muscle cell apical constriction cardiac muscle cell apical plasma membrane
Stage Range:	Protruding-mouth to Day 4

Phenotype Detail

Acknowledgments

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