FIGURE

Fig. S3

ID

ZDB-FIG-161219-22

Publication

Kaizuka et al., 2016 - An Autophagic Flux Probe that Releases an Internal Control

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Fig. S3

Induction of autophagy in fertilized zebrafish eggs (related to Figure 4)

(A) A schematic diagram of a fertilized zebrafish egg and GFP-LC3 signals in a GFP-LC3-RFP-LC3ΔG transgenic zebrafish egg at the indicated time (min post fertilization (mpf)). GFP-LC3 puncta appeared throughout the egg except for the yolk from approximately 12 mpf. The indicated regions are magnified in the insets. Scale bar, 10 μm and 1 μm (insets).

(B) Representative fluorescence and DIC images of fertilized eggs of wild-type and GFP-LC3-RFP-LC3ΔG transgenic zebrafish treated with or without 500 nM wortmannin at 1 and 2 h post fertilization. The graph shows the GFP/RFP fluorescence ratio in the blastomeres as a percentage relative to that of wortmannintreated eggs at 1 hpf. The indicated regions are shown and magnified in the insets. Scale bar, 5 μm. Data are representative of two independent experiments.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Mol. Cell