ZFIN Image: Kaizuka et al., 2016, Fig. S3

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Figure Caption

Fig. S3

Induction of autophagy in fertilized zebrafish eggs (related to Figure 4)

(A) A schematic diagram of a fertilized zebrafish egg and GFP-LC3 signals in a GFP-LC3-RFP-LC3ΔG transgenic zebrafish egg at the indicated time (min post fertilization (mpf)). GFP-LC3 puncta appeared throughout the egg except for the yolk from approximately 12 mpf. The indicated regions are magnified in the insets. Scale bar, 10 μm and 1 μm (insets).

(B) Representative fluorescence and DIC images of fertilized eggs of wild-type and GFP-LC3-RFP-LC3ΔG transgenic zebrafish treated with or without 500 nM wortmannin at 1 and 2 h post fertilization. The graph shows the GFP/RFP fluorescence ratio in the blastomeres as a percentage relative to that of wortmannintreated eggs at 1 hpf. The indicated regions are shown and magnified in the insets. Scale bar, 5 μm. Data are representative of two independent experiments.

Acknowledgments

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