FIGURE

Fig. 5

ID

ZDB-FIG-161219-21

Publication

Kaizuka et al., 2016 - An Autophagic Flux Probe that Releases an Internal Control

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Fig. 5

Spatial Analysis of Basal Autophagic Flux In Vivo in Live Zebrafish

(A and B) A schematic diagram of 1 dpf zebrafish and representative images of GFP and RFP fluorescence signals and DIC in the eye (lens and retina) (A) and tail (skeletal muscle and spinal cord) (B) of FIP200^+/+ and FIP200^−/− zebrafish embryos injected with GFP-LC3-RFP-LC3ΔG mRNA. Images of the indicated regions are shown in the insets. Scale bar, 10 μm.

(C) The graph shows the GFP/RFP fluorescence ratio of the indicated tissues of control (FIP200^+/+, FIP200^+/−, n = 3) and FIP200^−/− (n = 4) zebrafish embryos injected with GFP-LC3-RFP-LC3ΔG mRNA. Values are expressed as a percentage relative to that of FIP200^−/− zebrafish lens. Data represent mean ± SEM. ^∗p < 0.05 and ^∗∗p < 0.005. Data are representatives of three independent experiments.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rb1cc1^jt1/jt1
Observed In:	lens autophagy myotome autophagy optic cup autophagy spinal cord autophagy
Stage:	Prim-5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Mol. Cell