ZFIN Image: Kaizuka et al., 2016, Fig. 5

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Figure Caption

Fig. 5

Spatial Analysis of Basal Autophagic Flux In Vivo in Live Zebrafish

(A and B) A schematic diagram of 1 dpf zebrafish and representative images of GFP and RFP fluorescence signals and DIC in the eye (lens and retina) (A) and tail (skeletal muscle and spinal cord) (B) of FIP200^+/+ and FIP200^−/− zebrafish embryos injected with GFP-LC3-RFP-LC3ΔG mRNA. Images of the indicated regions are shown in the insets. Scale bar, 10 μm.

(C) The graph shows the GFP/RFP fluorescence ratio of the indicated tissues of control (FIP200^+/+, FIP200^+/−, n = 3) and FIP200^−/− (n = 4) zebrafish embryos injected with GFP-LC3-RFP-LC3ΔG mRNA. Values are expressed as a percentage relative to that of FIP200^−/− zebrafish lens. Data represent mean ± SEM. ^∗p < 0.05 and ^∗∗p < 0.005. Data are representatives of three independent experiments.

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Phenotype details

Acknowledgments

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