Fig. 5

Hair bundle density in the utricle. (A) Schematic of the utricle showing one sampling location in the extrastriolar region (box 1) and three locations in the striolar region (boxes 2–4, striolar region in light yellow). The phalloidin-labeled confocal image on the right depicts a striolar location. All boxes are 50 × 50 μm and are drawn to scale. (B) For lines made on a *AB background, bundle density is significantly different between genotypes and utricular locations (2-way ANOVA, F_5,189 = 8.36, p < 0.001; and F_3,189 = 89.39, p < 0.001, respectively). Posthoc comparisons of transgenic lines to *AB wildtype fish show a significant difference between *AB and Brn3c fish at utricular position 4 (*p < 0.05). (C) There is no significant difference in utricular hair bundle density between background strains (2-way ANOVA, F_2,59 = 2.64, p = 0.08 for background as a main effect). (D) Utricular hair bundle density does not differ between ET4 fish and the TL background strain on which they were created (2-way ANOVA, F_1,24 = 0.21, p = 0.65). Data are presented as mean ± S.E. N = 4–15 fish per group. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)