FIGURE

Fig. S2

ID

ZDB-FIG-160923-7

Publication

Qiu et al., 2016 - Embryonic hematopoiesis in vertebrate somites gives rise to definitive hematopoietic stem cells

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Fig. S2

GFP expression pattern in Tg(rbfox1l:gfp) transgenic line. (A) Confocal image showing GFP expression in somites of a Tg(rbfox1l:gfp) transgenic embryo at 22s stage. (B) The expression pattern of endogenous rbfox1l in wild-type embryo at 28 hpf, revealed by in situ hybridization. (C-C′′) GFP expression in somites of a transgenic embryo at 28 hpf (C). The enlarged trunk region (C′) and an optical cross section (C′′) were shown. (D) GFP+ circulating hematopoietic cells (indicated by an arrow) in the heart were observed by confocal microscopy at 36 hpf. (E) Flow cytometry result showing the existence of GFP⁺ blood cells in the embryonic circulation. Embryonic blood cells were sucked from the hearts of 5-10 embryos. A representative result was shown with the average (Av) from 3 independent experiments in parenthesis.

Expression Data

Genes:	EGFP rbfox1l
Fish:	TU rbfox1l^tsu054Gt
Anatomical Terms:	blood cell heart tube hematopoietic cell somite
Stage Range:	20-25 somites to Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Mol. Cell Biol.