FIGURE

Fig. S10

ID

ZDB-FIG-160923-15

Publication

Qiu et al., 2016 - Embryonic hematopoiesis in vertebrate somites gives rise to definitive hematopoietic stem cells

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Fig. S10

Blockage of primitive erythropoiesis by trim33 knockdown has little effect on somite hematopoiesis. (A) Effect of trim33 knockdown on primitive erythropoiesis. Wild-type embryos at the one-cell stage were injected with the control MO (cMO) or trim33-MO and collected at 30 hpf for detection of hemoglobin by O-dianisidine staining. The embryos were ventrally viewed with anterior to the left. The ratio of embryos with the representative pattern was indicated. Note that the number of red-blood cells was drastically reduced in trim33 morphants, indicating an effective knockdown. (B and C) Effect of trim33 knockdown on LPM (B) and somite hematopoiesis (C). Tg(gata1a:DsRed) (B) or Tg(foxc1b:EOS) (C) embryos at the one-cell stage were injected with corresponding MOs. For injected Tg(foxc1b:EOS) embryos, five pairs of new born somites were photoactivated at 20s stage. The circulating blood cells taken from a group of 5 embryos at 30 hpf were sorted by flow cytometry and the number of DsRed⁺ (B) or red-EOS⁺ cells (C) per embryo was averaged from three independent experiments. *, statistically significant with p < 0.05); ns, non-significance, p > 0.05.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Mol. Cell Biol.