ZFIN Image: Qiu et al., 2016, Fig. S10

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Figure Caption

Fig. S10

Blockage of primitive erythropoiesis by trim33 knockdown has little effect on somite hematopoiesis. (A) Effect of trim33 knockdown on primitive erythropoiesis. Wild-type embryos at the one-cell stage were injected with the control MO (cMO) or trim33-MO and collected at 30 hpf for detection of hemoglobin by O-dianisidine staining. The embryos were ventrally viewed with anterior to the left. The ratio of embryos with the representative pattern was indicated. Note that the number of red-blood cells was drastically reduced in trim33 morphants, indicating an effective knockdown. (B and C) Effect of trim33 knockdown on LPM (B) and somite hematopoiesis (C). Tg(gata1a:DsRed) (B) or Tg(foxc1b:EOS) (C) embryos at the one-cell stage were injected with corresponding MOs. For injected Tg(foxc1b:EOS) embryos, five pairs of new born somites were photoactivated at 20s stage. The circulating blood cells taken from a group of 5 embryos at 30 hpf were sorted by flow cytometry and the number of DsRed⁺ (B) or red-EOS⁺ cells (C) per embryo was averaged from three independent experiments. *, statistically significant with p < 0.05); ns, non-significance, p > 0.05.

Acknowledgments

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