FIGURE

Fig. 5

ID

ZDB-FIG-160811-22

Publication

Fujii et al., 2016 - Smyd5 plays pivotal roles in both primitive and definitive hematopoiesis during zebrafish embryogenesis

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Fig. 5

Expression of markers for definitive hematopoietic lineages in smyd5 morphants by WISH.

(A) Expression of cmyb in embryos injected with smyd5-MO1, smyd5-MO2, control embryos injected with Con-MO, and those no-injection at 30 hpf. Expression of mpx (B) and l-plastin (C) in embryos injected with smyd5-MO1, smyd5-MO2, control embryos injected with Con MO, and those no-injection at 72 hpf. Expression of hbbe1 (D) and rag1 (E) in embryos injected with smyd5-MO1, smyd5-MO2, control embryos injected with Con-MO, and those no-injection at 96 hpf. The number of embryos with the representative phenotype per the total number of embryos is indicated in each panel. Embryos are depicted in the lateral view. Scale bar, 200 µm.

Expression Data

Genes:	hbbe1.1 lcp1 mpx myb rag1
Fish:	WT WT + MO2-smyd5 WT + MO3-smyd5
Knockdown Reagents:	MO2-smyd5 MO3-smyd5
Anatomical Terms:	granulocyte lymphocyte macrophage nucleate erythrocyte ventral wall of dorsal aorta hematopoietic stem cell
Stage Range:	Prim-15 to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-smyd5 WT + MO3-smyd5
Knockdown Reagents:	MO2-smyd5 MO3-smyd5
Observed In:	granulocyte macrophage ventral wall of dorsal aorta hematopoietic stem cell
Stage Range:	Prim-15 to Protruding-mouth

Phenotype Detail

Acknowledgments

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